Petersson M, Pettersen JS, Henriksen HB, Duzs Á, Fernández-Quintero ML, Burlet NJ, Mojica N, Krengel U, Jenkins TP, Ward AB, Andersen TE, Møller-Jensen J, Gram L, Laustsen AH, Thrane SW.
Gut Microbes
Dec. 31, 2026
Bacterial enteric pathogens are major contributors to the global burden of diarrheal diseases and the associated consequences for human health including malnutrition, growth stunting, morbidity, and mortality. While mortality from diarrhea has decreased, incidence remains high, and better interventions for preventing disease are needed. Single-domain antibodies (i.e., VHHs), functioning as target-binding proteins in the gastrointestinal tract, have been proposed as a potential approach to mitigate bacterial pathogenesis. Here, we describe a mitigation strategy where precision binding of a bivalent VHH to the receptor-binding B-pentamer of heat-labile enterotoxin aggregates the AB5 toxin and impairs enterotoxigenic Escherichia coli colonization in a flow chamber model simulating the human intestine. The VHH construct also binds to the structurally similar cholera toxin and effectively abrogates its intestinal cell cytotoxicity in vitro. Based on these results, we believe that targeting virulence could emerge as a new strategy for the management of bacterial enteric pathogens, supporting gut health in at-risk populations alongside vaccination campaigns or in populations without access to vaccines.
Dueker K, Capozzola T, Feng Z, Lin RN, Hurtado J, Bangaru S, Yuan M, Beutler N, Garcia E, He WT, Callaghan S, Avillion G, Vo L, Li X, Torres JL, Musharrafieh R, Song G, Mishra N, Sharma P, Yong P, Anzanello F, Kaczmarek Michaels K, Ben-Akiva E, Silva M, Melo M, Makhdoomi M, Westfall-Gomez E, Rinaldi W, Ferguson M, Safonova Y, Crotty S, Irvine DJ, Rogers T, Ward AB, Briney B, Wilson IA, Burton DR, Andrabi R.
Li SH, Wang B, Jo G, Mikelov A, Atkinson RK, Le Sage V, Furey C, Ort JT, Ye N, Gang S, Shah R, Santos JJS, Röltgen K, Joshi SA, Lee JY, Pursell TA, Drapeau EM, Han J, Callear AP, Collman RG, Monto AS, Martin ET, Lakdawala SS, Ward AB, Wilson IA, Boyd SD, Hensley SE.
Fox A, Alafaci A, Carolan L, Burrell R, Sánchez-Ovando S, Hadiprodjo AJ, McMinn A, Macartney K, León AN, Ward AB, Wheatley A, Sullivan SG, Crawford NW, Britton PN, Subbarao K.
Sun J, Jo G, Troxell CA, Fu Y, Hoezl R, Lv H, Abozeid HH, Teo QW, Pholcharee T, McGrath JJC, Changrob S, Nelson SA, Yasuhara A, Huang M, Zheng NY, Chervin JC, Li L, Fernández-Quintero ML, Loeffler JR, Rodriguez AJ, Huang J, Swanson OM, Balmaseda A, Kuan G, Campredon L, Kaitlynn Allen E, Neumann G, Wu NC, Kawaoka Y, Krammer F, Mejias A, Ramilo O, Thomas PG, Gordon A, Ward AB, Han J, Wilson PC.
De Rosa SC, Gravett RM, Hahn WO, Villaran M, Huang Y, Schmitzberger L, Montefiori D, Domin E, Tomaras GD, Heptinstall J, Seaton KE, Ferrari G, Ozorowski G, Ward AB, Lee WH, van der Maas L, Polakowski L, Frank I, Tieu HV, Kalams SA, Garcia NMG, Huang J, Ghosh S, Purwar M, Kulp D, Humeau L, Kwong PD, McElrath MJ, Corey L, Weiner D, HVTN 304 Study Team.
Wu NR, Castro KM, Beutler N, Lee WH, Raghavan SSR, Martin GM, Jain M, Agrawal S, Liguori A, Kalyuzhniy O, Skog PD, Terada S, Lai YC, Ndihokubwayo J, Lu D, Eskandarzadeh S, Alavi N, Phelps N, Tingle R, Youhanna JE, Amirzehni S, Rogers TF, Burton DR, Wilson IA, Ward AB, Correia BE, Schief WR.
Schuhmacher JS, Xiao S, Eray ER, Brown S, Zambrowski A, Jain A, Garcia DM, Ozorowski G, Zhu W, Saam K, Caniels TG, Moore JP, Crispin M, Sanders RW, Chakraborty S, Correia BE, Ward AB, Antanasijevic A.
Liu J, Gang S, Kikawa C, Rodriguez AJ, Li SH, Ye N, Griffiths T, Drapeau EM, Atkinson RK, Loes AN, Collman RG, Ferguson JA, Han J, Ward AB, Bloom JD, Hensley SE.